Highest surf clam p120 mRNA levels coincide with the onset of neuronal growth. Unlike clam p 53 and p97, p120 mRNA and protein levels are temporally expressed in embryos, with mRNA levels decreasing with increasing p120 protein (R(2) = 0.97). These vertebrate forms of p 53 are required for normal inflammatory, epithelial, and neuronal development. The predicted protein, p120, resembles p 53 across all evolutionarily conserved regions and contains a C-terminal extension with a sterile alpha motif (SAM) as in p63 and p73. We sequenced, cloned, and characterized p120 cDNA. In these mollusks, p 53 and p97 occur in both embryonic and adult tissue, whereas p120 is exclusively embryonic. To study invertebrate p 53 during early development, we identified three differentially expressed p 53 family members ( p 53, p97, p120) in the surf clam, Spisula solidissima. The cell-cycle checkpoint protein p 53 both directs terminal differentiation and protects embryos from DNA damage. Jessen-Eller, Kathryn Kreiling, Jill A Begley, Gail S Steele, Marjorie E Walker, Charles W Stephens, Raymond E Reinisch, Carol L We propose that once the symbiosis is initiated, a symbiont-induced signal activates p 53 family members, inducing apoptosis and developmental morphogenesis of the light organ.Ī new invertebrate member of the p 53 gene family is developmentally expressed and responds to polychlorinated biphenyls. scolopes suggested that the p 53 family members are activated in cells of the symbiont-harvesting structures of the symbiotic light organ. Immunocytochemistry and Western blots using an antibody specific for E. These transcripts shared identical nucleotides where they overlapped, suggesting that they are splice variants of the same gene. Using degenerate RT-PCR and RACE PCR, we identified two p63-like transcripts encoding proteins of 431 and 567 amino acids. scolopes and described their role in symbiont-induced morphogenesis. In this study, we characterized p63-like transcripts from mRNA isolated from the symbiotic tissues of E. These proteins have been shown to induce apoptosis and developmental morphogenesis. Members of the p 53 family, including p 53, p63, and p73, are conserved across broad phyletic lines and p63 is thought to be the ancestral gene. Interactions with the symbiont result in the loss of a complex ciliated epithelium dedicated to promoting colonization of host tissue, and some or all of this loss is due to widespread, symbiont-induced apoptosis. Within hours of hatching, the squid Euprymna scolopes forms a specific light organ symbiosis with the marine luminous bacterium Vibrio fischeri. Goodson, Michael S Crookes-Goodson, Wendy J Kimbell, Jennifer R McFall-Ngai, Margaret J Characterization and role of p 53 family members in the symbiont-induced morphogenesis of the Euprymna scolopes light organ.
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